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In this paper, the problem of controlling the dissolved oxygen level (DO) during an aerobic fermentation is considered. The proposed approach deals with three major difficulties in respect to the nonlinear dynamics of the DO, the poor accuracy of the empirical models for the oxygen consumption rate and the fact that only sampled measurements are available on-line. A nonlinear integral high-gain control law including a continuous-discrete time observer is designed to keep the DO in the neighborhood of a set point value without any knowledge on the dissolved oxygen consumption rate. The local stability of the control algorithm is proved using Lyapunov tools. The performance of the control scheme is first analyzed in simulation and then experimentally evaluated during a successfull fermentation of the bacteria over a period of three days. Pseudomonas putida mt-2
To master complexity, we can organize it or discard it. The Art of Insight in Science and Engineering first teaches the tools for organizing complexity, then distinguishes the two paths for discarding complexity: with and without loss of information. Questions and problems throughout the text help readers master and apply these groups of tools. Armed with this three-part toolchest, and without complicated mathematics, readers can estimate the flight range of birds and planes and the strength of chemical bonds, understand the physics of pianos and xylophones, and explain why skies are blue and sunsets are red.
